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Phospho-peptide enrichment was performed using TiO 2 (Titansphere Phos-TiO Bulk 10 um, GL Science), ratio of beads to sample 10:1, mixed for 30 minutes at room temperature in 80% acetonitrile, 6% trifluoroacetic acid, 5 mM monopotassium phosphate, 20 mg/mL 2.5-dihydroxybenzoic acid

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Understanding these patterns helps practices set realistic expectations for patients and prioritize PA efforts where they are most likely to succeed

You may experience rapid appetite return, mood changes, and gastrointestinal shifts that make the adjustment unpleasant